The HPTLC is an effective tool to verify the fingerprint of identity of plant materials against a reference plate. We are able to confirm the profile of a plant thanks to this tool, and to ensure the right material is being used.
With an HPTLC, individual components of a mixture are separated on a thin glass-coated plate, which is then placed in a developing chamber. The TLC plate is placed under an ultraviolet lamp, and bands of the different components are visualized. Unlike a standard TLC, with an HPTLC many tasks are automated via robotics, eliminating uncertainty from samples being applied to plates by hand.
We use the HPTLC to detect contamination by mycotoxins, a dangerous class of toxins that can develop on plant matter in humid conditions.
You can see an HPTLC plate below.