Streamlining DNA purification, the QIACube automates this once-tedious process for DNA extraction in test samples before GMO testing. This automated system ensures reproducible results with minimal handson time, reducing human errors and enhancing process confidence.
Our laboratory has also added DNA extraction and analysis capability. With this capability we can identify if an ingredient was harvested from a genetically modified source.
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eDNA analysis (Environmental DNA)
Environmental DNA (eDNA) analysis is a technique that makes it possible to study the prevalence of different plant, fungi, and animal species in the environment through the DNA they shed in it.
DNA residues can be extracted from environmental samples including water, sediment, or soil. The samples are collected into the filters according to the separately agreed sampling plan. After extraction, polymerase chain reaction (PCR) is used to increase the abundance of DNA in the sample. The DNA is then sequenced using automatic sequencer instruments and compared to a database of known DNA sequences to identify the species it originated from.
Examples of eDNA testing applications include:
- Monitoring the presence of endangered or invasive species within a habitat.
- Evaluating soil quality by studying the prevalence of indicator species, such as microbes and fungi.
- Gathering data for an environmental impact assessment before a large-scale infrastructure project to minimize harm to vulnerable species.
The price of the analysis depends on the target species and the extensiveness of the project. Please describe these in detail when requesting an offer.
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Additional info CHATGPT – need to check 🙂
DNA extraction and purification are fundamental steps in molecular biology and genetics, and they are crucial for obtaining high-quality DNA for downstream applications such as PCR, sequencing, cloning, and other molecular analyses. Here is a general overview of the DNA extraction and purification process:
DNA Extraction:
- Sample Collection:
- Start with the collection of the biological material containing DNA. This could be cells, tissues, blood, or other sample types.
- Cell Lysis:
- Break open the cells to release the cellular components, including DNA. This can be achieved through mechanical disruption, enzymatic digestion, or chemical lysis.
- Protein Denaturation:
- Use a protein denaturing agent (such as SDS) to disrupt protein-DNA interactions and separate DNA from proteins.
- RNA Digestion (Optional):
- Treat the sample with RNase to remove RNA contaminants if RNA-free DNA is required.
- DNA Precipitation:
- Add a solution containing alcohol (usually isopropanol or ethanol) to precipitate the DNA from the solution.
- DNA Pellet Collection:
- Centrifuge the sample to collect the precipitated DNA into a pellet at the bottom of the tube.
- Wash and Resuspend:
- Wash the DNA pellet with ethanol to remove impurities, and then resuspend the DNA in an appropriate buffer.
DNA Purification:
- Column-Based Purification (Spin Columns):
- Use commercial DNA purification kits with spin columns containing silica membranes or other materials with affinity for DNA.
- Bind DNA to the column, wash away contaminants, and then elute purified DNA.
- Phenol-Chloroform Extraction (Organic Extraction):
- Perform phenol-chloroform extraction to remove proteins and other contaminants. This method requires careful handling of hazardous chemicals.
- Ethanol Precipitation:
- Precipitate DNA by adding ethanol or isopropanol and centrifuging to collect the DNA pellet. This method is often used after organic extraction or as an alternative to column-based purification.
- Gel Electrophoresis (Optional):
- Run an agarose gel electrophoresis to check the size and purity of the extracted DNA. This step is optional but can be valuable for quality control.
Additional Tips:
- Quality Control:
- Assess DNA concentration and purity using spectrophotometry (e.g., UV-Vis) or fluorometry.
- Check the integrity and size of DNA fragments through agarose gel electrophoresis or other methods.
- Storage:
- Store purified DNA at -20°C or -80°C for long-term preservation.
- Automation:
- High-throughput laboratories may employ automated systems for DNA extraction and purification to increase efficiency and reduce variability.
Always follow the specific protocols provided with the DNA extraction/purification kits or methods you choose, as they can vary based on the sample type and downstream applications.
